COURSE DESCRIPTION

Introduces general principles and application of specific instrumental methods. Emphasizes practical analysis of general laboratory and industrial substances. Lecture 2 hours. Laboratory 3 hours. Total 5 hours per week.

GENERAL COURSE PURPOSE

This course is designed to introduce the student to the common techniques of chemical instrumental analysis. The course is required for the student in the Semiconductor Laboratory Process programs, and may be appropriate for the student who wishes to earn an Associate in Science degree and to transfer to a four-year institution. It may be used as an elective for the student in health science, biotechnology, and environmental or industrial science fields.

ENTRY LEVEL COMPETENCIES

The prerequisite for this course is CHM 112 – "College Chemistry II".

COURSE OBJECTIVES

As a result of the learning experiences provided in this course, the student should be able to:

A. describe or define the theoretical basis for a instrumental design
B. compare and contrast instrumental designs
C. calibrate instruments and determine magnitudes and sources of error
D. analyze error using common statistical means
E. determine propagation of error

A. Simple circuits

1. Voltage measurements
2. Transducers
3. Operational amplifiers
4. Analog-to-digital converters

B. Error Analyses

1. Mean, median, range, standard deviations
2. Tests for data rejection
3. Propagation of error

C. Absorption Technique—UV/VIS/IR—Electromagnetic Radiation

1. Sources, cells, monochromators, detectors
2. Single and double beam instruments—output format
3. Beer’s law and deviations: mixtures
4. Sources of error
5. Fine structure of VIS/IR: rotational and vibrational spectra
6. Atomic absorption: emission techniques

D. Chromatography

1. Principles and parameters
2. Factors affecting retention times
3. Gas-liquid chromatography (GLC)
4. High-pressure liquid chromatography (HPLC)
5. Quantitative analysis

E. Electrochemistry

1. Standard potentials and thermodynamics
2. Nernstian behavior and concentration determination
3. Ion selective electrodes
4. Potentiometric titrations
5. Coulometry: end point detection
6. Direct, cyclic and Pulsed Voltammetry

F. Magnetic Resonance 1. Nuclear

• ¹H first order/shielding and coupling
• ¹H second order: decoupling, shift reagents frequency effects
• ¹³C, ¹⁹F, ³¹P, and other nuclei
• medical applications

2. Electron

• Analogies to NMR
• Qualitative analysis
• High resolution instruments and elemental analysis

G. Mass Spectrometry

1. Fundamentals
2. Qualitative analysis
3. High resolution instruments and elemental analysis